Difference between revisions of "Part:BBa K4241792"

 
 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4241792 short</partinfo>
 
<partinfo>BBa_K4241792 short</partinfo>
 +
<partinfo>BBa_K4241792 SequenceAndFeatures</partinfo>
  
−
mCherry-XL is a basic (constitutively fluorescent) red fluorescent protein published in 2022, derived from Discosoma sp.. It has low acid sensitivity.
+
===Overview===
 +
 
 +
mCherry-XL is a basic red fluorescent protein published in 2022, derived from Discosoma sp.. This RFP is evolved to have increased lifespan, increased quantum yield to be on par as the brightest RFP.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
mCherry-XL is derived from a lifetime-based evolution of mCherry. Four positions were identified near the FP chromophore to be mutated to increase lifetime, resulting in the derivative mCherry-XL (eXtended Lifetime). This RFP boasts an impressive 3-fold quantum yield matching the brightest RFP to date mScarlet.
 +
<br/>
 +
This is a gene to be used as a reporter. This can be done via fluorescent microscopy.
 +
 +
===References===
 +
Mukherjee, S., Manna, P., Hung, S.-T., Vietmeyer, F., Friis, P., Palmer, A., &amp; Jimenez, R. (2022). Directed evolution of a bright variant of mcherry: Suppression of non-radiative decay by fluorescence lifetime selections. https://doi.org/10.26434/chemrxiv-2022-v3t03
  
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K4241792 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 09:46, 10 October 2022


mCherry XL RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Overview

mCherry-XL is a basic red fluorescent protein published in 2022, derived from Discosoma sp.. This RFP is evolved to have increased lifespan, increased quantum yield to be on par as the brightest RFP.

Usage and Biology

mCherry-XL is derived from a lifetime-based evolution of mCherry. Four positions were identified near the FP chromophore to be mutated to increase lifetime, resulting in the derivative mCherry-XL (eXtended Lifetime). This RFP boasts an impressive 3-fold quantum yield matching the brightest RFP to date mScarlet.
This is a gene to be used as a reporter. This can be done via fluorescent microscopy.

References

Mukherjee, S., Manna, P., Hung, S.-T., Vietmeyer, F., Friis, P., Palmer, A., & Jimenez, R. (2022). Directed evolution of a bright variant of mcherry: Suppression of non-radiative decay by fluorescence lifetime selections. https://doi.org/10.26434/chemrxiv-2022-v3t03