Difference between revisions of "Part:BBa K4229015"
Line 4: Line 4:
  
  
−
XiaI is a novel terpenoid cyclase, which was taken out of the xiamycin gene cluster in Streptomyces sp. XiaI turnes indol into either 3-Oxindole or 2-Oxindole. XiaI workes better with the help of Fre(BBa_K4229001) wich helps to recycle NADHP and provides hydrogen to the cyclase.
+
XiaI is a novel terpenoid cyclase, which was taken out of the xiamycin gene cluster in Streptomyces sp. XiaI turnes indol into either 3-Oxindole or 2-Oxindole. XiaI workes better with the help of Fre(BBa_K4229001) wich helps to recycle NADHP and provides hydrogen to the cyclase. This biobrick consists of XiaI,which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project.
  
 
[[File:Grafik indigo weier bg.png|900px|thumb|left|]]
 
[[File:Grafik indigo weier bg.png|900px|thumb|left|]]

Revision as of 05:40, 10 October 2022


spyCatcherXiaI


XiaI is a novel terpenoid cyclase, which was taken out of the xiamycin gene cluster in Streptomyces sp. XiaI turnes indol into either 3-Oxindole or 2-Oxindole. XiaI workes better with the help of Fre(BBa_K4229001) wich helps to recycle NADHP and provides hydrogen to the cyclase. This biobrick consists of XiaI,which is fused with the snoopcatcher(BBa_K4229009) on the N-terminal of the protein. With that, we made it able to be recruted into the wiffelball we used in our project.

Grafik indigo weier bg.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]