Difference between revisions of "Part:BBa K215107"
Line 4: Line 4:
 
This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5
 
This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5
  
−
Used to secrete proteins containing prtB C-terminus tag, expression vector BBa_K215002 should be used[[https://parts.igem.org/Part:BBa_K215002]].
+
Used to secrete proteins containing prtB C-terminus tag, expression vector BBa_K215002 [[https://parts.igem.org/Part:BBa_K215002]] should be used.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:32, 1 October 2009

Extracellular Secretion System

This is the 3 genes: prtD, prtE, prtF, that are from a type I Erwinia chrysanthemi secretion system. The operon in under expression by a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5

Used to secrete proteins containing prtB C-terminus tag, expression vector BBa_K215002 [[1]] should be used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1487
    Illegal BsaI.rc site found at 1133
    Illegal BsaI.rc site found at 3737