Difference between revisions of "Part:BBa K4158008"
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Cyanuric acid, the final metabolite of atrazine, has a specific LysR-type Transcription regulator (LTTR), AtzR. | Cyanuric acid, the final metabolite of atrazine, has a specific LysR-type Transcription regulator (LTTR), AtzR. | ||
This part encodes RBS and coding site of AtzR protein. | This part encodes RBS and coding site of AtzR protein. | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
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Upon the binding of cyanuric acid, AtzR activates transcription by altering its binding sites to the operon through conformational change. | Upon the binding of cyanuric acid, AtzR activates transcription by altering its binding sites to the operon through conformational change. | ||
Thus, GFP downstream of the binding region of AtzR on the reporter plasmid ([https://parts.igem.org/Part:BBa_K4158004 BBa_K4158004]) is expressed under the presence of cyanuric acid [1]. | Thus, GFP downstream of the binding region of AtzR on the reporter plasmid ([https://parts.igem.org/Part:BBa_K4158004 BBa_K4158004]) is expressed under the presence of cyanuric acid [1]. | ||
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[[File:Waseda Tokyo The mechanism of GFP expression.png|300px|thumb|center|Fig.1. The mechanism of GFP expression]] | [[File:Waseda Tokyo The mechanism of GFP expression.png|300px|thumb|center|Fig.1. The mechanism of GFP expression]] | ||
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| + | ===Results=== | ||
In order to detect cyanuric acid in the cell-free protein synthesis system in <i>E.coli</i>, we transformed this part into <i>BL21(DE3)Star</i> strain and prepared crude extracts which were pre-enriched with the transcription factor, AtzR [2]. | In order to detect cyanuric acid in the cell-free protein synthesis system in <i>E.coli</i>, we transformed this part into <i>BL21(DE3)Star</i> strain and prepared crude extracts which were pre-enriched with the transcription factor, AtzR [2]. | ||
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Revision as of 02:25, 10 October 2022
RBS-AtzR
Cyanuric acid, the final metabolite of atrazine, has a specific LysR-type Transcription regulator (LTTR), AtzR. This part encodes RBS and coding site of AtzR protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 415
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 415
Illegal NotI site found at 701 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 415
Illegal BamHI site found at 151 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 415
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 415
- 1000COMPATIBLE WITH RFC[1000]