Difference between revisions of "Part:BBa K4497011"

 
 
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<partinfo>BBa_K4497011 short</partinfo>
 
<partinfo>BBa_K4497011 short</partinfo>
  
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This part encodes the intracellular protease function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see [[Part:Bba_K4497017]]).
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==Design & Cloning==
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===Design===
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This part is made of the following components:
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* CD 28 transmembrane domain ([[Part:BBa_K4497004]]): transmembrane anchor for the MESA system
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* Tobacco Etch Virus (TEV) Protease ([[Part:BBa_K4497005]]): Protease cleaving proteins the TEV recognition site
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===Cloning===
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*G1TA: [[Part:BBa_K4497017]]
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*G1TEV: [[Part:BBa_K4497018]]
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*M1TA: [[Part:BBa_K4497019]]
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*M1TEV: [[Part:BBa_K4497020]]
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*G2TA: [[Part:BBa_K4497021]]
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*G2TEV: [[Part:BBa_K4497022]]
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*M2TA: [[Part:BBa_K4497023]]
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*M2TEV: [[Part:BBa_K4497024]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:07, 13 October 2022


TEV MESA Part

This part encodes the intracellular protease function of the MESA system. We used it in combination with mCherry and GFP nanobodies (see Part:Bba_K4497017).

Design & Cloning

Design

This part is made of the following components:

  • CD 28 transmembrane domain (Part:BBa_K4497004): transmembrane anchor for the MESA system
  • Tobacco Etch Virus (TEV) Protease (Part:BBa_K4497005): Protease cleaving proteins the TEV recognition site

Cloning

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 448
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 448
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 448
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]