Difference between revisions of "Part:BBa K4147010"
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<partinfo>BBa_K4147010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4147010 SequenceAndFeatures</partinfo> | ||
| − | ===Characterization of | + | ===Characterization of RFP construct with LacI regulated promoter for bacterial expression=== |
<center>[[File:MCherry-Tec-Chihuahua.png |300px]]</center> | <center>[[File:MCherry-Tec-Chihuahua.png |300px]]</center> | ||
| − | <center><b>Figure 1</b>. SnapGene®️ map of BioBrick | + | <center><b>Figure 1</b>. SnapGene®️ map of BioBrick BBa_K4147010.</center> |
| + | |||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4147010 parameters</partinfo> | <partinfo>BBa_K4147010 parameters</partinfo> | ||
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Revision as of 23:28, 9 October 2022
mCherry (RFP) construct with LacI regulated promoter for bacterial expression
This mCherry reporter is one of several "second-generation" monomeric fluorescent proteins. The proposed construct for its expression it's conformed by a LacI regulated promoter (BBa_R0010), an RBS (BBa_B0032) and a double terminator (BBa_B0015). Lastly this construct was optimized for its production on E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization of RFP construct with LacI regulated promoter for bacterial expression
