Difference between revisions of "Part:BBa K4447005"
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The usage of genetic constructs that are designed to produce a protein, either inside or outside a cell, is important in several ways, such as increasing the expression rate. With this BioBrick, we are generating a <b>bacterial expression platform</b> that is capable of generating several copies of our protein of interest, <i>EryK</i>, coupled with the FRET system for the detection of erythromycin.  
 
The usage of genetic constructs that are designed to produce a protein, either inside or outside a cell, is important in several ways, such as increasing the expression rate. With this BioBrick, we are generating a <b>bacterial expression platform</b> that is capable of generating several copies of our protein of interest, <i>EryK</i>, coupled with the FRET system for the detection of erythromycin.  
  
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Our expression platform is composed of the following elements: an <b>inducible promoter</b>, which is regulated in response to specific stimuli; a <b>ribosome-binding site</b> to bind the ribosome for the initiation of translation; and a <b>transcription terminator<b> that mediates the release of the transcript RNA from the translational complex.
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Our expression platform is composed of the following elements: an <b>inducible promoter</b>, which is regulated in response to specific stimuli; a <b>ribosome-binding site</b> to bind the ribosome for the initiation of translation; and a <b>transcription terminator</b> that mediates the release of the transcript RNA from the translational complex.

Revision as of 22:52, 9 October 2022


Bacterial Expression System for the Detection of Erythromycin based on FRET

Expression system based on FRET biosensor for the detection of erythromycin. This system is suitable for cloning in pSB1C3 as the plasmid backbome, which propagates the BioBrick part and is widely used by other iGEM teams.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3812
    Illegal SapI site found at 961


Usage and Biology

The usage of genetic constructs that are designed to produce a protein, either inside or outside a cell, is important in several ways, such as increasing the expression rate. With this BioBrick, we are generating a bacterial expression platform that is capable of generating several copies of our protein of interest, EryK, coupled with the FRET system for the detection of erythromycin.

Our expression platform is composed of the following elements: an inducible promoter, which is regulated in response to specific stimuli; a ribosome-binding site to bind the ribosome for the initiation of translation; and a transcription terminator that mediates the release of the transcript RNA from the translational complex.