Difference between revisions of "Part:BBa K4497008"

Revision as of 13:51, 13 October 2022

GFP Nanobody Receptor

This part encodes the seqeunce for a GFP-nanobody based receptor for secretion/ surface expression. The receptor contains a myc-tag for immunofluorescence labeling.

Design & Cloning

Design

This part is made of the following components:

IL-11 Signaling Sequence (Part:BBa_K4497003): Signaling Sequence for Cell surface expression
Myc-tag (Part:BBa_K112502): Myc epitope tag that enables surface expression detection using immunofluorescence labeling
Anti-GFP nanobody (Part:BBa_K4497002): a nanobody capable of binding GFP

Cloning

The part is encoded on the plasmid pcDNA3.1-myc-GFPnb-gp130 which was kindly provided to us by Prof. Scheller [1]

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 349
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 349
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 103
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 349
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 349
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4497008 short</partinfo>
-v
+This part encodes the seqeunce for a GFP-nanobody based receptor for secretion/ surface expression. The receptor contains a myc-tag for immunofluorescence labeling.
+==Design & Cloning==
+===Design===
+This part is made of the following components:
+* IL-11 Signaling Sequence ([[Part:BBa_K4497003]]): Signaling Sequence for Cell surface expression
+* Myc-tag ([[Part:BBa_K112502]]): Myc epitope tag that enables surface expression detection using immunofluorescence labeling
+* Anti-GFP nanobody ([[Part:BBa_K4497002]]): a nanobody capable of binding GFP
+===Cloning===
+The part is encoded on the plasmid  pcDNA3.1-myc-GFPnb-gp130 which was kindly provided to us by Prof. Scheller [1]
 <!-- Add more about the biology of this part here