Difference between revisions of "Part:BBa K4294807"

Latest revision as of 14:58, 12 October 2022

TU pLuxr PhlF

Design

We used this transcriptional unit in our Positive Feedback+ Repressor (BBa_K4294804) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs. PhlF was chosen for our constructs since it is an effective repressor with a low association constant K, indicating its ability to form functional dimers in lower concentrations (according to the Ecoli Marionette strains characterization [1])

Reference

[1] Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26. PMID: 30478458.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4294807 short</partinfo>
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+==Design==
-<!-- Add more about the biology of this part here
+We used this transcriptional unit in our Positive Feedback+ Repressor (BBa_K4294804) and Positive Feedback with constitutive LuxR production + Repressor (BBa_K4294805) constructs. PhlF was chosen for our constructs since it is an effective repressor with a low association constant K, indicating its ability to form functional dimers in lower concentrations (according to the Ecoli Marionette strains characterization [1])
-===Usage and Biology===
+==Reference==
+[1] Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26. PMID: 30478458.
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