Difference between revisions of "Part:BBa K4147014"

Revision as of 19:11, 9 October 2022

Reverse of BBa_K525998.

This part is used for the expression of siRNA. It consists in the reves¿rse sequence of a T7 promoter and a RBS.

In order to produce double-stranded siRNAs, we need the sequences in both directions. This is why we designed the reverse sequence for this part.

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K4147014 short</partinfo>
-This part is used for the expression of siRNA. In order to produce double-stranded siRNAs, we need the sequences in both directions. This is why we designed the reverse sequence for this part.
+This part is used for the expression of siRNA. It consists in the reves¿rse sequence of a T7 promoter and a RBS.
+In order to produce double-stranded siRNAs, we need the sequences in both directions. This is why we designed the reverse sequence for this part.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+To produce the double-stranded siRNAs, an expression system has been designed and must be assembled.
+The assembly method is based on the one shown by the <b>iGEM Ecuador 2021 team</b>.
+The figure below shows the assembly strategy that is planned to be used.
+[[File:Up_RXLR.png|600px|thumb|center|alt=action.|Assembly strategy for siRNA production]]
 <!-- -->