Difference between revisions of "Part:BBa K245007"
 
Line 2: Line 2:
 
<partinfo>BBa_K245007 short</partinfo>
 
<partinfo>BBa_K245007 short</partinfo>
  
−
This plasmid backbone is the same as [[BBa_K245004]] except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004.
+
This plasmid backbone is the same as BBa_K245004 except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:27, 30 September 2009

BB-NIC-II-HisC

This plasmid backbone is the same as BBa_K245004 except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2082
    Illegal XbaI site found at 2150
    Illegal SpeI site found at 13
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2082
    Illegal SpeI site found at 13
    Illegal PstI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2082
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2082
    Illegal XbaI site found at 2150
    Illegal SpeI site found at 13
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2082
    Illegal XbaI site found at 2150
    Illegal SpeI site found at 13
    Illegal PstI site found at 40
    Illegal NgoMIV site found at 2156
    Illegal AgeI site found at 2162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1121