Difference between revisions of "Part:BBa K4160007"

 
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IL-10 is an anti-inflammatory cytokine. It is used as the therapeutic agent of our project.
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<p>Interleukin 10 (IL-10) (Figure 1) is an anti-inflammatory cytokine, that has an essential role during infections. IL-10 inhibits the immune response during inflammation, to prevent damaging the host.<sup>1</sup> IL-10 is not on the white list. However, the TU-Eindhoven 2022 team received permission from the Safety and Security Committee to implement the use of this part.</p><br>
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<figure><img src="https://static.igem.org/mediawiki/parts/b/b6/BBa_K4160007_TU-Eindhoven_IL-10_structure.png" width="400px" heigth=320px">
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<p><b>Figure 1 | 3D structure of IL-10.</b> (PDB: 1ILK)<sup>2</sup></p>
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
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<partinfo>BBa_K4160007 SequenceAndFeatures</partinfo>
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<h2>Usage and Biology</h2>
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<p>IL-10 originates from the <i>Homo sapiens (human)</i> and is the primary immune regulatory cytokine that has thorough anti-inflammatory functions while operating on many immune cells.<sup>2</sup> IL-10 is primarily produced by monocytes and minorly by lymphocytes.<sup>3</sup> It suppresses the expression of MHC class II proteins, Th1 cytokines, and costimulatory molecules on macrophages. Furthermore, IL-10 can enhance B-cell survival, proliferation, and production of antibodies.<sup>3</sup> IL-10 is recognized to have potential therapeutic effects on inflammatory diseases, including autoimmune diseases.<sup>4</sup></p><br>
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<h3>Role in autoimmune diseases</h3>
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<p>Since IL-10 lowers the inflammatory host response, it is co-responsible for preventing inflammation in autoimmune pathologies.<sup>5</sup> Hence, IL-10 deficiency or dysregulation is acknowledged to cause several autoimmune diseases. Therefore, the powerful anti-inflammatory characteristics of IL-10 suggest the therapeutic potential of IL-10 administration in autoimmune diseases.<sup>5</sup></p><br>
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<h3>GEMS system</h3>
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<p><a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>). Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 will act as the therapeutic agent to treat ANCA-associated vasculitis.</p><br>
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<h2>Characterization</h2>
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<p>Characterization of IL-10 expression by the GEMS system can be found on the <a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a> page.</p><br><br>
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<h2>References</h2>
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<li>Saraiva M, O’Garra A. The regulation of IL-10 production by immune cells. Nat Rev Immunol. 2010;10(3):170-181. doi:10.1038/NRI2711</li>
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<li>IL10 - Interleukin-10 - Homo sapiens (Human) | UniProtKB | UniProt. Accessed October 11, 2022. https://www.uniprot.org/uniprotkb/P22301/entry#structure</li>
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<li>IL10 interleukin 10 [Homo sapiens (human)] - Gene - NCBI. Accessed October 11, 2022. https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3586%5Buid%5D</li>
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<li>Tian G, Li JL, Wang DG, Zhou D. Targeting IL-10 in Auto-immune Diseases. Cell Biochem Biophys. 2014;70(1):37-49. doi:10.1007/S12013-014-9903-X/TABLES/1</li>
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<li>Iyer SS, Cheng G. Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune Disease. Crit Rev Immunol. 2012;32(1):23. doi:10.1615/CRITREVIMMUNOL.V32.I1.30</li>
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Revision as of 14:10, 11 October 2022


IL-10 (interleukin 10)

Interleukin 10 (IL-10) (Figure 1) is an anti-inflammatory cytokine, that has an essential role during infections. IL-10 inhibits the immune response during inflammation, to prevent damaging the host.1 IL-10 is not on the white list. However, the TU-Eindhoven 2022 team received permission from the Safety and Security Committee to implement the use of this part.


Figure 1 | 3D structure of IL-10. (PDB: 1ILK)2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

IL-10 originates from the Homo sapiens (human) and is the primary immune regulatory cytokine that has thorough anti-inflammatory functions while operating on many immune cells.2 IL-10 is primarily produced by monocytes and minorly by lymphocytes.3 It suppresses the expression of MHC class II proteins, Th1 cytokines, and costimulatory molecules on macrophages. Furthermore, IL-10 can enhance B-cell survival, proliferation, and production of antibodies.3 IL-10 is recognized to have potential therapeutic effects on inflammatory diseases, including autoimmune diseases.4


Role in autoimmune diseases

Since IL-10 lowers the inflammatory host response, it is co-responsible for preventing inflammation in autoimmune pathologies.5 Hence, IL-10 deficiency or dysregulation is acknowledged to cause several autoimmune diseases. Therefore, the powerful anti-inflammatory characteristics of IL-10 suggest the therapeutic potential of IL-10 administration in autoimmune diseases.5


GEMS system

TU-Eindhoven 2022 used this part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system (BBa_K4160008). Upon activation of the GEMS receptor, STAT-induced IL-10 will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, IL-10 will act as the therapeutic agent to treat ANCA-associated vasculitis.


Characterization

Characterization of IL-10 expression by the GEMS system can be found on the BBa_K4160008 page.



References

  1. Saraiva M, O’Garra A. The regulation of IL-10 production by immune cells. Nat Rev Immunol. 2010;10(3):170-181. doi:10.1038/NRI2711
  2. IL10 - Interleukin-10 - Homo sapiens (Human) | UniProtKB | UniProt. Accessed October 11, 2022. https://www.uniprot.org/uniprotkb/P22301/entry#structure
  3. IL10 interleukin 10 [Homo sapiens (human)] - Gene - NCBI. Accessed October 11, 2022. https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=3586%5Buid%5D
  4. Tian G, Li JL, Wang DG, Zhou D. Targeting IL-10 in Auto-immune Diseases. Cell Biochem Biophys. 2014;70(1):37-49. doi:10.1007/S12013-014-9903-X/TABLES/1
  5. Iyer SS, Cheng G. Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune Disease. Crit Rev Immunol. 2012;32(1):23. doi:10.1615/CRITREVIMMUNOL.V32.I1.30