Difference between revisions of "Part:BBa K4286100"

Revision as of 14:40, 9 October 2022

Effector device in timed suicide switch

2022 SZU-China plans to design a timed suicide switch mediated by a classical synthetic gene oscillator. The core of the effector is the MazEF toxin-antitoxin system, in which the antitoxin mazE is controlled by the Promoter tetR , and the antitoxin mazF is controlled by the constitutive promoter PJ23110. The MazEF system is an important component of the subtilisin-antitoxin system, which leads to programmed cell death. MazF is an mRNA endonuclease that specifically cleaves a five-base UACAU sequence on RNA. In the presence of MazE, MazF has a higher affinity for MazE than its RNA substrate. MazE binds to MazF to occupy its active site, rendering it unable to bind or cleave the mRNA.

Usage and Biology

The core of the effector is the MazEF toxin-antitoxin system from Bacillus subtilis, which can lead to programmed cell death. The antitoxin MazE is controlled by the Promoter tetR, and the toxin MazF is controlled by the constitutive Promoter PJ23110. MazF is an endonuclease that specifically cleaves UACAU sites on mRNA. MazE combines with MazF at a ratio of 1:1 to occupy its active site and make it lose its toxicity. The effector is encoded in the high-copy plasmid colE1.

Assembly

modeling

Sequencing

Sequence and Features