Difference between revisions of "Part:BBa K239000:Design"

(→‎References)
(→‎Design Notes)
Line 8: Line 8:
 
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI.  
 
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI.  
 
Only one SpeI restriction site had to be removed.
 
Only one SpeI restriction site had to be removed.
−
Some uncertainty about where to start and stop in the sequence. It starts 3 bp before the first CpxR binding site and ends 6 pb after the -10 box.
+
The sequence starts 3 bp before the first CpxR binding site.
  
 
===Source===
 
===Source===

Revision as of 16:06, 28 September 2009

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only one SpeI restriction site had to be removed. The sequence starts 3 bp before the first CpxR binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References

Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System Kunihiko Nishino, Takeshi Honda, and Akihito Yamaguchi J Bacteriol. 2005 March; 187(5): 1763–1772. doi: 10.1128/JB.187.5.1763-1772.2005. PMCID: PMC1063996


Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli Stéphanie Bury-Moné, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931