Difference between revisions of "Part:BBa K4137003"
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This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality. | This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality. | ||
+ | ===Construct Design=== | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 03:17, 10 October 2022
Chi18h8-YebF Fusion Protein
This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality.
Construct Design
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1492
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1005
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 552
- 1000COMPATIBLE WITH RFC[1000]