Difference between revisions of "Part:BBa K4343129"
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<partinfo>BBa_K4343129 short</partinfo> | <partinfo>BBa_K4343129 short</partinfo> | ||
− | + | A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 85%. | |
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Latest revision as of 11:49, 10 October 2022
GAP-nCBE
A cytosine base editor with GAP promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 85%.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1679
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5003
Illegal BamHI site found at 3958 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 489
Illegal AgeI site found at 567 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 570
Illegal BsaI site found at 5581
Illegal BsaI.rc site found at 564
Illegal BsaI.rc site found at 4690
Illegal BsaI.rc site found at 5845