Difference between revisions of "Part:pSB1AT3"

 
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pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
 
pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
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===Usage and Biology===
 
===Usage and Biology===
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Tetracycline resistant up to 150 ug/ml.
 
Tetracycline resistant up to 150 ug/ml.
  
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<partinfo>pSB1AT3 SequenceAndFeatures</partinfo>
 
<partinfo>pSB1AT3 SequenceAndFeatures</partinfo>
  
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>pSB1AT3 parameters</partinfo>
 
<partinfo>pSB1AT3 parameters</partinfo>
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Revision as of 02:17, 5 June 2006


High copy BioBrick assembly plasmid

pSB1AT3 is a high copy number plasmid carrying ampicillin and tetracycline resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).


Usage and Biology

Tetracycline resistant up to 150 ug/ml.

Kanamycin sensitive at 5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal NheI site found at 1270
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3431
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3425
    Illegal BamHI site found at 1416
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 2318
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3425
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3425
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3440
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1442
    Illegal NgoMIV site found at 1810
    Illegal NgoMIV site found at 1970
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2464