Difference between revisions of "Part:BBa K4156083"
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φ174E induces bacteria lysis in our constructed effector strains, thereby releasing intracellularly expressed therapeutic proteins.
 
φ174E induces bacteria lysis in our constructed effector strains, thereby releasing intracellularly expressed therapeutic proteins.
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===Characterization===
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We used φ174E in the E. coli lysis experiment with validation of therapeutic efficacy, and characterization could be seen at  <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156100"> BBa_K4156100 </a></html> ;  <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156105"> BBa_K4156105 </a></html> ；  <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156109"> BBa_K4156109 </a></html>.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:42, 11 October 2022


φ174E

φ174E is a kind of phage cleavage gene, which can trigger the death of the bacteria.

Usage and Biology

φ174E induces bacteria lysis in our constructed effector strains, thereby releasing intracellularly expressed therapeutic proteins.

Characterization

We used φ174E in the E. coli lysis experiment with validation of therapeutic efficacy, and characterization could be seen at BBa_K4156100  ; BBa_K4156105 ； BBa_K4156109 .



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]