Difference between revisions of "Part:BBa K4137012"
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The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). When malate binds to the p_mleS promoter, it induces the production of CcdA, the antitoxin which assures chi18h8’s production around the plant roots. | The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). When malate binds to the p_mleS promoter, it induces the production of CcdA, the antitoxin which assures chi18h8’s production around the plant roots. | ||
− | [File: pmles-ccda-myc.png|800px|thumb|center|Fig.1 Construct design for ccdA toxin secretion.]] | + | [[File: pmles-ccda-myc.png|800px|thumb|center|Fig.1 Construct design for ccdA toxin secretion.]] |
===Construct Design=== | ===Construct Design=== |
Revision as of 06:20, 9 October 2022
CcdA antitoxin secretion pathway
The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). When malate binds to the p_mleS promoter, it induces the production of CcdA, the antitoxin which assures chi18h8’s production around the plant roots.
Construct Design
We attached a Myc epitope Tag downstream of the ccdA sequence for purification purposes. A p_mleS promoter is attached upstream to the side of the open reading frame (ccdB-Myc coding complex) and RBS (AAGGAG). The terminator BBa_B1006 is attached downstream of the sequence.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]