Difference between revisions of "Part:BBa K4343095"
Line 5: | Line 5: | ||
EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2. | EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2. | ||
− | + | ====Characterization==== | |
− | + | ||
− | + | ||
− | + | ||
− | |||
− | |||
The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6). | The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6). | ||
+ | |||
+ | ====Reference==== | ||
+ | |||
+ | 1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z. | ||
+ | 12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010). | ||
+ | |||
+ | |||
+ | |||
+ | |||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Line 22: | Line 26: | ||
<partinfo>BBa_K4343095 parameters</partinfo> | <partinfo>BBa_K4343095 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
− | |||
− | |||
− |
Latest revision as of 06:19, 9 October 2022
EgElo9
EgElo9 encodes ∆-9 elongase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.
Characterization
The EgElo9 gene encodes a protein of 258 amino acids. Heterologous expression of EgElo9 could catalyze linoleic acid (LA, C18:2) to eicosadienoic acid (EDA; C20: 2n-6).
Reference
1. Zhu, Q. 365, 307–316 (1999). et al. Metabolic engineering of an oleaginous yeast for the production of omega-3 fatty acids. in Single Cell Oils: Microbial and Algal Oils (eds. Cohen, Z. 12. & Ratledge, C.) 51–73 (AOCS press, Urbana, 2010).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 354
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 794