Difference between revisions of "Part:BBa K4137000:Design"
 
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The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli.
 
The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli.
 
An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein.
 
An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein.
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[Image:ccda-his.png|750px|thumb|center|Figure 1. ccdA with a C terminal 6xHis Tag.]]
 
  
 
===Source===
 
===Source===

Latest revision as of 03:06, 9 October 2022


CcdA-6xHis Antitoxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli. An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein.

Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1

References

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1