Difference between revisions of "Part:BBa K4147003"
Line 11: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4147003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4147003 SequenceAndFeatures</partinfo>
 +
 +
===Characterization of PcOSM Construct with LacI regulated promoter===
 +
<center>[[File:PcOSM_Construct_with_LacI_regulated_promoter.png |300px]]</center>
 +
<center><b>Figure 1</b>. SnapGene®️ map of BioBrick BBa_K4147003.</center>
  
  

Revision as of 21:32, 9 October 2022


PcOSM Construct with LacI regulated promoter

This part contains the linear construct for pcOSM (plant osmotin from Piper colubrinum) with an included LacI promoter. Additionally, it contains an RBS under the part name BBa_B0032, as well as a 6X-His tag at the end of the osmotin CDS for later protein purification. Finally, it has a double terminator (BBa_B0015) to ensure correct termination. As a linear construct, it contains the RFC10 prefix and suffix, making the part compatible with other iGEM parts.


Usage and Biology

This osmotin is a plant defense protein with a molecular weight of ~24 kDa. PcOSM belongs to pathogenesis related-5 (PR-5) family, which is accumulated in response to both biotic and abiotic stresses [1]. This osmotin has exhibited significant inhibitory activity against the oomycete pathogen Phytophthora capsici. This protein is able to inhibit fungal growth through inhibition of hyphal growth, spore germination, spore lysis, and reduction in viability of spore [2]. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization of PcOSM Construct with LacI regulated promoter

PcOSM Construct with LacI regulated promoter.png
Figure 1. SnapGene®️ map of BioBrick BBa_K4147003.


REFERENCES

[1] Geetha, R. G., Krishnankutty Nair Chandrika, S., Saraswathy, G. G., Nair Sivakumari, A., & Sakuntala, M. (2021). ROS Dependent Antifungal and Anticancer Modulations of Piper colubrinum Osmotin. Molecules, 26(8), 2239. doi:10.3390/molecules26082239

[2] Chowdhury, S., Basu, A., & Kundu, S. (2015). Cloning, Characterization, and Bacterial Over-Expression of an Osmotin-like Protein Gene from Solanum nigrum L. with Antifungal Activity Against Three Necrotrophic Fungi. Molecular Biotechnology, 57(4), 371–381. doi:10.1007/s12033-014-9831-4