Difference between revisions of "Part:BBa K4174003:Design"

Revision as of 22:23, 8 October 2022

Design Notes

Monomeric red fluorescence protein, or mRFP1, is a monomer, has a rapid maturity rate, and has minimal spectral overlap with GFP compared to the wild-type red fluorescent protein DsRed (Campbell et al. 2002). However, other DsRed variants have a much higher fluorescence quantum yield and extinction coefficient than mRFP1 (Campbell et al. 2002).

Source

Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q. A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., & Endy, D. (2013). Precise and reliable gene expression via standard transcription and translation initiation elements. Nature methods, 10(4), 354–360. https://doi.org/10.1038/nmeth.2404

References

Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A., & Tsien, R. Y. (2002). A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 7877–7882. https://doi.org/10.1073/pnas.082243699

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 ===Design Notes===
-Monomeric red fluorescence protein, or mRFP1 is a monomer, has a rapid maturity rate, and has minimal spectral overlap with GFP compared to the wild-type red fluorescent protein DsRed (Campbell <i>et al.</i> 2002). However, other DsRed variants have a much higher fluorescence quantum yield and extinction coefficient than mRFP1 (Campbell <i>et al.</i> 2002).
+Monomeric red fluorescence protein, or mRFP1, is a monomer, has a rapid maturity rate, and has minimal spectral overlap with GFP compared to the wild-type red fluorescent protein DsRed (Campbell <i>et al.</i> 2002). However, other DsRed variants have a much higher fluorescence quantum yield and extinction coefficient than mRFP1 (Campbell <i>et al.</i> 2002).
 ===Source===