Difference between revisions of "Part:BBa K4165254"
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<partinfo>BBa_K4165254 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165254 SequenceAndFeatures</partinfo> | ||
− | + | ===WetLab Results=== | |
+ | <p style=" font-weight: bold; font-size:14px;"> Transfotmation of GST COH in BL-21 using pGS-21a vector </p> | ||
+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh.jpg" style="margin-left:200px;" alt="" width="500" /></p> | ||
+ | </html> | ||
+ | Figure 2. Transformed plate of GST COH + pGS-21a | ||
+ | <p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH in DH-5 alpha using pJET vector </p> | ||
+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p> | ||
+ | </html> | ||
+ | Figure 3. Transformed plate of GST COH + pJET | ||
+ | <p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH </p> | ||
+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/gst-coh2.jpg" style="margin-left:200px;" alt="" width="500" /></p> | ||
+ | </html> | ||
+ | Figure 4. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4165254 parameters</partinfo> | <partinfo>BBa_K4165254 parameters</partinfo> | ||
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Revision as of 15:38, 11 October 2022
GST-Coh2
This part encodes Cohesin 2 protein tagged with GST for its purification and characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
WetLab Results
Transfotmation of GST COH in BL-21 using pGS-21a vector
Figure 2. Transformed plate of GST COH + pGS-21a
Transformation of GST COH in DH-5 alpha using pJET vector
Figure 3. Transformed plate of GST COH + pJET
Comparison between chemical lysis and sonication for GST COH
Figure 4. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH