Difference between revisions of "Part:BBa K4043004"

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<b>Characterization</b>
 
<b>Characterization</b>
 
<p>To measure activity of this part, we created the measurement part <b><partinfo>BBa_K4202029</partinfo></b> and then constructed it to the  vector pHY300PLK and transformed it to <i>Bacillus subtilis</i> WB600 strain. The bacteria containing the measurement plasmid were than cultured in the TB media and induced by different concentrations of sucrose and sucralose(a sucrose analogue).</p>
 
<p>To measure activity of this part, we created the measurement part <b><partinfo>BBa_K4202029</partinfo></b> and then constructed it to the  vector pHY300PLK and transformed it to <i>Bacillus subtilis</i> WB600 strain. The bacteria containing the measurement plasmid were than cultured in the TB media and induced by different concentrations of sucrose and sucralose(a sucrose analogue).</p>
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[[File:ZJU-China2022-PsacB measurement result.png]]

Revision as of 17:09, 8 October 2022


A weak promoter

The transcription of the PsacB promoter is not strictly regulated by sucrose, and can be transcribed without an inducer, but the intensity is 100 times lower than that of sucrose induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

Contribution

Group: ZJU-China 2022
Author: Zhijian Yan

Summary: In 2022, ZJU-China characterized this part and used this part in their project to design a kill switch regulated by the concentration of sucrose(BBa_K4202045). The characterization result indicated the promoter activity is too low to permit reliable characterization result when using GFP as reporter. The kill switch containing this promoter has been proved to work very well in Bacillus subtilis WB600 strain.


Characterization

To measure activity of this part, we created the measurement part BBa_K4202029 and then constructed it to the vector pHY300PLK and transformed it to Bacillus subtilis WB600 strain. The bacteria containing the measurement plasmid were than cultured in the TB media and induced by different concentrations of sucrose and sucralose(a sucrose analogue).

File:ZJU-China2022-PsacB measurement result.png