Difference between revisions of "Part:BBa K4268005:Experience"
 
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[[File: T-suny-oneonta-t7-tail tubular protein B colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Tail Tubular Protein B insert. The insert length is 2751bp and the predicted size of the PCR product when using VF/VR primers is 3056bp.]]
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[[File: T-suny-oneonta-t7-tail tubular protein B colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Tail Tubular Protein B insert. The insert length is 2751bp and the predicted size of the PCR product when using VF/VR primers is 3056bp.]]
  
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Tail Tubular Protein B was successfully cloned into a Level 0 Golden Gate Assembly basic part.
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Tail Tubular Protein B was successfully cloned into a Level 0 Golden Gate Assembly basic part.

Latest revision as of 18:22, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of containing the Tail Tubular Protein B insert. The insert length is 2751bp and the predicted size of the PCR product when using VF/VR primers is 3056bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Tail Tubular Protein B was successfully cloned into a Level 0 Golden Gate Assembly basic part.

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