Difference between revisions of "Part:BBa K4244010"

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<partinfo>BBa_K4244010 short</partinfo>
 
<partinfo>BBa_K4244010 short</partinfo>
  
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The <partinfo>BBa_B0010</partinfo> terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that cause for a high protein expression.  
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Strong terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that cause for a high protein expression.
  
 
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<partinfo>BBa_K4244010 parameters</partinfo>
 
<partinfo>BBa_K4244010 parameters</partinfo>
 
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===References===
 
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Chapter 5 of thesis Thijs Nieuwkoop, Translational feed-forward andfeed-back control, Sjoerd C.A. Creutzburg*,Thijs Nieuwkoop*, Thijmen Zegers & John van der Oost.
 

Revision as of 13:00, 9 October 2022


Terminator with 3' spacer region (#9) for high protein production

Strong terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that cause for a high protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]