Difference between revisions of "Part:BBa K4361005"
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| − | + | BlcR is a transcription factor originating from the bacterium <i>Agrobacterium tumefaciens</i> ([[Part:BBa_K4361100]]). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs (IRs), short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' (IR1) and 'ATTAGttgaactCTAAT' (IR2), as further explained in [[Part:BBa_K4361001]]. <br> | |
| + | The original IR1 sequence is not a perfect reverse complement of itself. This part and [[Part:BBa_K4361004]] have been designed to test whether or not exchanging IR1 for one of two perfect reverse complement sequences would increase its binding strength to BlcR. To create this part, the nucleotides on the 5' half of IR1 have been replaced by the reverse complement of those of the 3' half, designated as RV 2. The BlcR-binding domain of this part thus consists of IR1 RV 2-tca-IR2, where tca is the original 3 nt linker sequence between IRs. | ||
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Revision as of 07:31, 10 October 2022
BlcR-binding oligo, 51 bp, IR1 perfect RV 2 + IR2
BlcR is a transcription factor originating from the bacterium Agrobacterium tumefaciens (Part:BBa_K4361100). In a homodimer state it contains a single DNA-binding domain that specifically binds one of two DNA sequences. Both sequences are so-called inverted repeat pairs (IRs), short DNA sequences whose ends are reverse complements of each other. For the Blc operator, these sequences are 'ACTCTAATgATTCAAGT' (IR1) and 'ATTAGttgaactCTAAT' (IR2), as further explained in Part:BBa_K4361001.
The original IR1 sequence is not a perfect reverse complement of itself. This part and Part:BBa_K4361004 have been designed to test whether or not exchanging IR1 for one of two perfect reverse complement sequences would increase its binding strength to BlcR. To create this part, the nucleotides on the 5' half of IR1 have been replaced by the reverse complement of those of the 3' half, designated as RV 2. The BlcR-binding domain of this part thus consists of IR1 RV 2-tca-IR2, where tca is the original 3 nt linker sequence between IRs.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and biology
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Results
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