Difference between revisions of "Part:BBa K4164016"
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<partinfo>BBa_K4164016 short</partinfo>
 
<partinfo>BBa_K4164016 short</partinfo>
  
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This  composite part was constructed to analyze the function of ddRFP and the  intensity of the T7 lac promoter.
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This  composite part was constructed to analyze the function of ddRFP and the  intensity of the T7 <em>lac</em> promoter.
  
 
The composite part can be directly imported into plasmid and express ddRFPA1-ddRFPB1 induced with IPTG.
 
The composite part can be directly imported into plasmid and express ddRFPA1-ddRFPB1 induced with IPTG.
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We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device.
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<p style="text-align: center;"><img src="https://static.igem.wiki/teams/4164/wiki/part-registry/part-004005016998ddrfpplate.png"with="1000" height="" width="500" height=""/></p>
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<p style="text-align: center!important;"><b>Fig.1 Fluorescence image of <em> E. coli</em> expressing ddRFPA1-ddRFPB1 and control.</b></p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:01, 12 October 2022


Inductive expression of ddRFPA1-ddRFPB1

This composite part was constructed to analyze the function of ddRFP and the intensity of the T7 lac promoter.

The composite part can be directly imported into plasmid and express ddRFPA1-ddRFPB1 induced with IPTG.

We connected ddRFPA1 and ddRFPB1 by a flexible linker(3*GGGGS) to verify the feasicility of ddRFPA1 and ddRFPB1. Following overnight incubation at 37℃, we can see the bright red fluorescence (Fig.1). In this case, we chose ddRFPA1 and ddRFPB1 as our report device.

Fig.1 Fluorescence image of E. coli expressing ddRFPA1-ddRFPB1 and control.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1271
    Illegal NheI site found at 1539
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]