Difference between revisions of "Part:BBa K4204016"

 
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<partinfo>BBa_K4204017 short</partinfo>
  
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This part contains a coding sequence of sfGFP (BBa_K515105) with N-terminal His tag from pET28a(+). It is intended to be used to characterize the crystal violet induction system in our project. The N-terminal His tag mimics the realistic protein expression condition of pET28a(+).
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4204017 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4204017 parameters</partinfo>
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Revision as of 12:36, 8 October 2022

N terminal His-tagged sfGFP

This part contains a coding sequence of sfGFP (BBa_K515105) with N-terminal His tag from pET28a(+). It is intended to be used to characterize the crystal violet induction system in our project. The N-terminal His tag mimics the realistic protein expression condition of pET28a(+).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]