Difference between revisions of "Part:BBa K4244002"
Line 3: Line 3:
 
<partinfo>BBa_K4244002 short</partinfo>
 
<partinfo>BBa_K4244002 short</partinfo>
  
 +
 +
The stop codon of sfGFP (<partinfo>BBa_K3143689</partinfo>) was removed to allow C-terminus fusion with signal peptide. <br>
 +
 +
A silent mutation was also performed to removed the SapI site present in <partinfo>BBa_K3143689</partinfo> at position 13 (A to G) to make it RFC[1000] compatible.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:26, 8 October 2022


sfGFP without stop codon


The stop codon of sfGFP (BBa_K3143689) was removed to allow C-terminus fusion with signal peptide.

A silent mutation was also performed to removed the SapI site present in BBa_K3143689 at position 13 (A to G) to make it RFC[1000] compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]