Difference between revisions of "Part:BBa K4193026"
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− | the | + | Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter. |
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+ | <meta charset="utf-8"> | ||
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+ | <body> | ||
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+ | <img src="https://static.igem.org/mediawiki/parts/1/17/QS.jpg" style="width: 80%;"> | ||
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+ | </body> | ||
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+ | Figure1.The signal pathway of IP-mediated quorum sensing | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 10:46, 12 October 2022
QS system
Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1092
Illegal EcoRI site found at 3483
Illegal EcoRI site found at 5357
Illegal EcoRI site found at 5682
Illegal XbaI site found at 2581
Illegal XbaI site found at 3040
Illegal XbaI site found at 3124
Illegal XbaI site found at 3487
Illegal XbaI site found at 6643
Illegal SpeI site found at 5194
Illegal PstI site found at 5444
Illegal PstI site found at 6143 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1092
Illegal EcoRI site found at 3483
Illegal EcoRI site found at 5357
Illegal EcoRI site found at 5682
Illegal SpeI site found at 5194
Illegal PstI site found at 5444
Illegal PstI site found at 6143 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1092
Illegal EcoRI site found at 3483
Illegal EcoRI site found at 5357
Illegal EcoRI site found at 5682
Illegal BglII site found at 523
Illegal BglII site found at 880
Illegal BglII site found at 1129
Illegal BglII site found at 2065
Illegal BglII site found at 2539
Illegal BglII site found at 4398
Illegal BamHI site found at 633 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1092
Illegal EcoRI site found at 3483
Illegal EcoRI site found at 5357
Illegal EcoRI site found at 5682
Illegal XbaI site found at 2581
Illegal XbaI site found at 3040
Illegal XbaI site found at 3124
Illegal XbaI site found at 3487
Illegal XbaI site found at 6643
Illegal SpeI site found at 5194
Illegal PstI site found at 5444
Illegal PstI site found at 6143 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1092
Illegal EcoRI site found at 3483
Illegal EcoRI site found at 5357
Illegal EcoRI site found at 5682
Illegal XbaI site found at 2581
Illegal XbaI site found at 3040
Illegal XbaI site found at 3124
Illegal XbaI site found at 3487
Illegal XbaI site found at 6643
Illegal SpeI site found at 5194
Illegal PstI site found at 5444
Illegal PstI site found at 6143
Illegal NgoMIV site found at 6410
Illegal NgoMIV site found at 6450
Illegal NgoMIV site found at 6490
Illegal NgoMIV site found at 6530
Illegal AgeI site found at 6420
Illegal AgeI site found at 6460
Illegal AgeI site found at 6500
Illegal AgeI site found at 6540 - 1000COMPATIBLE WITH RFC[1000]