Difference between revisions of "Part:BBa K4255014"
| Line 1: | Line 1: | ||
| + | We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS sequences come from (四个物种). We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS. | ||
| + | The function of the enzymes can be listed below: | ||
F3H: catalyze flavanone into dihydrokaempferol | F3H: catalyze flavanone into dihydrokaempferol | ||
F3’5’H: catalyze dihydrokaempferol into dihydromyricetin | F3’5’H: catalyze dihydrokaempferol into dihydromyricetin | ||
| + | DFR: catalyze dihydromyricetin into delphinin colorless | ||
| + | ANS: catalyze delphinin colorless into delphinidin | ||
Revision as of 10:40, 8 October 2022
We divided the seven enzymes into two parts, 4CL, CHS and CHI catalyzed the production of naringenin one by one, and naringenin was further generated into anthocyanins in the presence of F3H, F3'5'H, DFR and ANS. The F3H, F3'5'H, DFR and ANS sequences come from (四个物种). We constructed the last four enzymes on the pRSFDuet vector, in which F3H was fused to F3'5'H and DFR was fused to ANS. The function of the enzymes can be listed below: F3H: catalyze flavanone into dihydrokaempferol F3’5’H: catalyze dihydrokaempferol into dihydromyricetin DFR: catalyze dihydromyricetin into delphinin colorless ANS: catalyze delphinin colorless into delphinidin