Difference between revisions of "Part:BBa K4165230"
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<partinfo>BBa_K4165230 short</partinfo>
 
<partinfo>BBa_K4165230 short</partinfo>
  
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Clamp [14] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGG (BBa_K4165018), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
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Clamp [15] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGG (BBa_K4165018), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
  
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
 
According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).
 
According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).
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===3D Modeling===
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TRRosetta models this composite part with a score of 5 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs).
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/15.png" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                              Figure 1. The 3D structure of Clamp 15 modeled by TRrosetta
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Revision as of 09:06, 8 October 2022


Clamp [15]

Clamp [15] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGG (BBa_K4165018), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]