Difference between revisions of "Part:BBa K4193011:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription. | + | we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. This Quorum-sensing systems were used to test the effect of IP concentration on the expression of target genes, and thus AtlPT4 was not available. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription. |
===Source=== | ===Source=== | ||
Latest revision as of 08:26, 8 October 2022
quorum sensing system (two parts)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. This Quorum-sensing systems were used to test the effect of IP concentration on the expression of target genes, and thus AtlPT4 was not available. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription.
Source
synthesize from company
References
Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.