Difference between revisions of "Part:BBa K4137003"

Revision as of 12:18, 9 October 2022

Chi18h8-YebF Fusion Protein

This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1492
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1005
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 552
1000
COMPATIBLE WITH RFC[1000]

Revision as of 05:54, 8 October 2022 (view source) Stuberosum ryan (Talk \| contribs) ← Older edit		Revision as of 12:18, 9 October 2022 (view source) Stuberosum ryan (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4137003 short</partinfo>		<partinfo>BBa_K4137003 short</partinfo>

−	This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed.	+	This part consists of the secretion tag YebF (BBa_K2922002), a GS Linker (BBa_J18921), a chi18h8 coding sequence, and a 6xHis tag. The sequence is reversed in our experimentation, but for documentation purposes, it is entered in the forward directionality.