Difference between revisions of "Part:BBa K4268002:Experience"
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[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid assembly protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 866 bp.]]
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[[File: T-suny-oneonta-t7-capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 866 bp.]]
  
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.
 
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.

Revision as of 05:46, 8 October 2022


The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.


Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert capsid protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 866 bp.

The gel indicates that all five colonies are likely to contain the correct insert and thus, the Capsid Protein was successfully cloned into a Level 0 Golden Gate Assembly basic part.

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