Difference between revisions of "Part:BBa K4268000:Experience"
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− | [[File: T-suny-oneonta-t7-head tail connector colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is | + | [[File: T-suny-oneonta-t7-head tail connector colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1878 bp.]] |
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level 0 Golden Gate Assembly basic part. | The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level 0 Golden Gate Assembly basic part. | ||
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<I>Username</I> | <I>Username</I> | ||
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− | Enter the review | + | Enter the review information here. |
|}; | |}; | ||
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Revision as of 16:21, 8 October 2022
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level 0 Golden Gate Assembly basic part.
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UNIQf5b7231d4e748fb2-partinfo-00000000-QINU UNIQf5b7231d4e748fb2-partinfo-00000001-QINU