Difference between revisions of "Part:BBa K4193004"
| Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter. | |
| + | <html> | ||
| + | <head> | ||
| + | <meta charset="utf-8"> | ||
| + | |||
| + | </head> | ||
| + | <body> | ||
| + | <div> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/1/17/QS.jpg" style="width: 80%;"> | ||
| + | </div> | ||
| + | </body> | ||
| + | </html> | ||
<!-- --> | <!-- --> | ||
Revision as of 10:44, 12 October 2022
SSRE promoter
Quorum sensing system related promoter
Usage and Biology
Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 239
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 239
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 239
Illegal NgoMIV site found at 6
Illegal NgoMIV site found at 46
Illegal NgoMIV site found at 86
Illegal NgoMIV site found at 126
Illegal AgeI site found at 16
Illegal AgeI site found at 56
Illegal AgeI site found at 96
Illegal AgeI site found at 136 - 1000COMPATIBLE WITH RFC[1000]