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a riboswitch regulated by a specific molecule
 
a riboswitch regulated by a specific molecule
  
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===Design===
 
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By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.
 
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<img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width: 80%;">
 
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===Usage===
 
===Usage===
 
The content of root target molecule controls the expression of target gene
 
The content of root target molecule controls the expression of target gene

Latest revision as of 04:52, 8 October 2022


ribozyme riboswitch

a riboswitch regulated by a specific molecule

Usage

The content of root target molecule controls the expression of target gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]