Difference between revisions of "Part:BBa K4193002"
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===Usage===
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The content of root target molecule controls the expression of target gene
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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===Usage===
 
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The content of root target molecule controls the expression of target gene
 
  
 
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Revision as of 04:31, 8 October 2022


ribozyme riboswitch

a riboswitch regulated by a specific molecule

Design

By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.

Usage

The content of root target molecule controls the expression of target gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]