Difference between revisions of "Part:BBa K4193002"
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a riboswitch regulated by a specific molecule | a riboswitch regulated by a specific molecule | ||
− | + | ===Design=== | |
− | === | + | By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence. |
− | + | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 04:22, 8 October 2022
ribozyme riboswitch
a riboswitch regulated by a specific molecule
Design
By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 38
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 38
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 38
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 38
- 1000COMPATIBLE WITH RFC[1000]