Difference between revisions of "Part:BBa K4137014"
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<partinfo>BBa_K4137014 short</partinfo> | <partinfo>BBa_K4137014 short</partinfo> | ||
− | The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The second half contains a T7 promoter, | + | The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The second half contains a T7 promoter, an RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This half produces and purifies mleR, the regulator of CcdA, which produces when malate acid is present. The construct as a whole code for Ccda is regulated by mleR when malate acid is presented. |
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Revision as of 10:03, 8 October 2022
CcdA regulatory and expression device
The mleR regulatory site which comes from p_mles binds with the RBS and CcdA, the antitoxin that will bind with CcdB when induced by malate acid and detoxifies it, the purification tag 6xHis, and the terminator B1006 (BBa_B1006). This sequence codes for CcdA, the antitoxin which assures chi18h8’s production around the plant roots. The second half contains a T7 promoter, an RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This half produces and purifies mleR, the regulator of CcdA, which produces when malate acid is present. The construct as a whole code for Ccda is regulated by mleR when malate acid is presented.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 419
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]