Difference between revisions of "Part:BBa K4137008"
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This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented. | This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented. | ||
− | + | [[File:t7-mler-his.png|800px|thumb|center|Fig.1 Malate-binding transcriptional activator + 6x His-Tag complete construct.]] | |
− | === | + | |
+ | ===Construct Designs=== | ||
+ | We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence. | ||
+ | |||
+ | ===Characterization=== | ||
+ | |||
+ | ===Sequence and Features=== | ||
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Revision as of 04:43, 9 October 2022
mleR expressing construct
This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.
Construct Designs
We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.
Characterization
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 49
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]