Difference between revisions of "Part:BBa K4146507"

Revision as of 02:54, 8 October 2022

pCS-aroGfbr

 aroG, as a metabolic pathway gene that has attracted much attention for many years, has always been a worthy object of study. We believe that its feedback inhibition may affect the synthesis of 2-phenylethyl alcohol. We use this Mutant Feedback-Resistant Version of aroG for to resisting feedback inhibition and improving enzyme activity. This is the composite of our Improvement of Standard Biological Parts.
 Our team successfully constructed a pathway for the synthesis of 2-phenylethanol in Escherichia coli. When cultured for 60 hours, the maximum concentration of 2-phenylethanol was 67.16mg/L.

The standard curve for determination of 2-phenylethanol yield

The experimental 2-phenylethanol yield

Sequence and Features