Difference between revisions of "Part:BBa K4268005:Experience"
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| − | [[File: T-suny-oneonta-t7- | + | [[File: T-suny-oneonta-t7-internal core protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Tail Tubular Protein B insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.]] |
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part. | The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part. | ||
Revision as of 03:56, 8 October 2022
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
File:T-suny-oneonta-t7-internal core protein colony PCR figure.png
Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Tail Tubular Protein B insert. The insert length is 1573bp and the predicted size of the PCR product when using VF/VR primers is 1739 bp.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
Applications of BBa_K4268005
User Reviews
UNIQ4b8dc3c5a6c4f571-partinfo-00000000-QINU UNIQ4b8dc3c5a6c4f571-partinfo-00000001-QINU