Difference between revisions of "Part:BBa K4477010:Design"
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===Source=== | ===Source=== | ||
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https://salislab.net/software/ | https://salislab.net/software/ | ||
===References=== | ===References=== | ||
Revision as of 22:35, 7 October 2022
RBS for camelid anti-mouse VHH expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used a pre-existing RBS, BBa_J61100, as a starting point for optimization with Salis. We aimed for 75% relative expression so as to get a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene. We optimized the RBS sequence to exclude restriction sites incompatible with various assembly methods.
Source
BBa_J61100 https://salislab.net/software/