Difference between revisions of "Part:BBa K4180003"
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C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br>
 
C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein<br><br>
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Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212<br><br>
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:36, 7 October 2022


snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212

Sequence and Features

       BBa K4180003 snf1Δ381-633aa
      PCR product on lane 3 compared to
       BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

        After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa
         PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a comparison



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]