Difference between revisions of "Part:BBa K4180004"
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The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project | The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project | ||
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(enhanced green fluorescent protein) is used as control | (enhanced green fluorescent protein) is used as control | ||
The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive<br><br> | The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive<br><br> | ||
Revision as of 17:20, 7 October 2022
enhanced GFP (eGFP)
The enhanced GFP will be used as a control. It will be made into a composite part, pGal1, 10-eGFP-SBP (BBa_K4180008) as a control in the project
(enhanced green fluorescent protein) is used as control
The original GFP from the jellyfish Aequorea victoria was not bright enough to show the gene expression or the protein localization of several different organisms. Yang et al lab did several mutations on the GFP DNA sequence to enhance the brightness of GFP to make the detection much more sensitive
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]