Difference between revisions of "Part:BBa K4180000"
Line 4: Line 4:
  
 
<h4>
 
<h4>
−
AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP,     and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)
+
<!-- AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP, and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)-->
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:06, 7 October 2022


SNF1 is the metabolism sensor

Sequence and Features

     BBa_K4180000 SNF1 full length
         PCR product

After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)
         PCR product




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]